Recombinant human CD14 protein

CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. Recombinant human CD14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14 molecule

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.9 kDa (353aa) confirmed by MALDI-TOF

Additionnal Information

CD14, monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, ATGP2697-10 µg, ATGP2697-20 µg, ATGP2697-50 µg, ATGP2697-100 µg, ATGP2697-250 µg, ATGP2697-500 µg, ATGP2697-1 mg, ATGP2697-10, ATGP2697-20, ATGP2697-50, ATGP2697-100, ATGP2697-250, ATGP2697-500, ATGP2697-1

References & Citations

Kelley S.L., et al (2013) . J. Immunol. 190:1304-1311; ; Setoguchi M., et al (1989) . Biochim. Biophys. Acta 1008:213-222;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.15M NaCl, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_001167576

Uniprot Accession Number

P08571

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSTTPEPCE LDDEDFRCVC NFSEPQPDWS EAFQCVSAVE VEIHAGGLNL EPFLKRVDAD ADPRQYADTV KALRVRRLTV GAAQVPAQLL VGALRVLAYS RLKELTLEDL KITGTMPPLP LEATGLALSS LRLRNVSWAT GRSWLAELQQ WLKPGLKVLS IAQAHSPAFS CEQVRAFPAL TSLDLSDNPG LGERGLMAAL CPHKFPAIQN LALRNTGMET PTGVCAALAA AGVQPHSLDL SHNSLRATVN PSAPRCMWSS ALNSLNLSFA GLEQVPKGLP AKLRVLDLSC NRLNRAPQPD ELPEVDNLTL DGNPFLVPGT ALPHEGSMNS GVV