Recombinant human CD14 protein
CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. Recombinant human CD14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14 molecule
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
37.9 kDa (353aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kelley S.L., et al (2013) . J. Immunol. 190:1304-1311; ; Setoguchi M., et al (1989) . Biochim. Biophys. Acta 1008:213-222;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.15M NaCl, 1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_001167576
Uniprot Accession Number
P08571
Species
Human
AA Sequence
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