Recombinant human DNMT3L protein

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. DNMT3L is a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. It also mediates transcriptional repression through interaction with histone deacetylase 1. Recombinant human DNMT3L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

DNA (cytosine-5) -methyltransferase 3-like isoform 2, DNA (cytosine-5-) -methyltransferase 3-like

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

46.2 kDa (411aa) confirmed by MALDI-TOF

Additionnal Information

DNMT3L, DNA (cytosine-5) -methyltransferase 3-like isoform 2, DNA (cytosine-5-) -methyltransferase 3-like, ATGP2696-10 µg, ATGP2696-20 µg, ATGP2696-50 µg, ATGP2696-100 µg, ATGP2696-250 µg, ATGP2696-500 µg, ATGP2696-1 mg, ATGP2696-10, ATGP2696-20, ATGP2696-50, ATGP2696-100, ATGP2696-250, ATGP2696-500, ATGP2696-1

References & Citations

Ooi S.K., Qiu C, et al. (2007) Nature 448:714-717;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_787063

Uniprot Accession Number

Q9UJW3

Species

Human

AA Sequence

MAAIP ALDPEAEPSM DVILVGSSEL SSSVSPGTGR DLIAYEVKAN QRNIEDICIC CGSLQVHTQH PLFEGGICAP CKDKFLDALF LYDDDGYQSY CSICCSGETL LICGNPDCTR CYCFECVDSL VGPGTSGKVH AMSNWVCYLC LPSSRSGLLQ RRRKWRSQLK AFYDRESENP LEMFETVPVW RRQPVRVLSL FEDIKKELTS LGFLESGSDP GQLKHVVDVT DTVRKDVEEW GPFDLVYGAT PPLGHTCDRP PSWYLFQFHR LLQYARPKPG SPRPFFWMFV DNLVLNKEDL DVASRFLEME PVTIPDVHGG SLQNAVRVWS NIPAIRSRHW ALVSEEELSL LAQNKQSSKL AAKWPTKLVK NCFLPLREYF KYFSTELTSS L