Recombinant human AFAP protein

AFAP1 is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human AFAP1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Actin filament associated protein 1, AFAP, AFAP-110

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

39.5 kDa (362aa)

Additionnal Information

AFAP, Actin filament associated protein 1, AFAP, AFAP-110, ATGP2693-10 µg, ATGP2693-20 µg, ATGP2693-50 µg, ATGP2693-100 µg, ATGP2693-250 µg, ATGP2693-500 µg, ATGP2693-1 mg, ATGP2693-10, ATGP2693-20, ATGP2693-50, ATGP2693-100, ATGP2693-250, ATGP2693-500, ATGP2693-1

References & Citations

Qian Y. et al. (2004) J Cell Biochem. 91: 602-620.; ; Qian Y. et al. (1998) Oncogene. 16:2185-2195.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Cytoskeleton

NCBI Accession Number

AAH32777.1

Uniprot Accession Number

Q8N556

Species

Human

AA Sequence

GCSGPVDSE CPPPPSSPVH KAELEKKLSS ERPSSDGEGV VENGITTCNG KEQVKRKKSS KSEAKGTVSK VTGKKITKII SLGKKKPSTD EQTSSAEEDV PTCGYLNVLS NSRWRERWCR VKDNKLIFHK DRTDLKTHIV SIPLRGCEVI PGLDCKHPLT FRLLRNGQEV AVLEASSSED MGRWIGILLA ETGSSTDPEA LHYDYIDVEM SASVIQTAKQ TFCFMNRRVI SANPYLGGTS NGYAHPSGTA LHYDDVPCIN GSLRGKKPPV ASNGVTGKGK TLSSQPKKAD PAAVVKRTGS NAAQYKYGKN RVEADAKRLQ TKEEELLKRK EALRNRLAQL RK