Recombinant human Uridine-cytidine kinase 1/UCK1 protein

UCK1 is a uridine-cytidine kinase that catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (uMP) and cytidine monophosphate (CMP) but not the phosphorylation of deoxyribonucleosides or purine ribonucleosides. This enzyme can also phosphorylate uridine and cytidine analogs and uses both ATP and GTP as a phosphate donor. Alternative splicing results in multiple splice variants encoding distinct isoforms. Recombinant human uCK1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

URK1, Cytidine monophosphokinase 1, Uridine monophosphokinase 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

17.5 kDa (152aa)

Additionnal Information

URK1, Uridine-cytidine kinase 1 isoform a, Uridine cytidine kinase 1 isoform a, Uridine monophosphokinase 1, uCK1, RP11-334J6.5, RP11 334J6.5, FLJ12255, Cytidine monophosphokinase 1, ATGP2692-10 µg, ATGP2692-20 µg, ATGP2692-50 µg, ATGP2692-100 µg, ATGP2692-250 µg, ATGP2692-500 µg, ATGP2692-1 mg, ATGP2692-010, ATGP2692-020, ATGP2692-050, ATGP2692-100, ATGP2692-250, ATGP2692-500, ATGP2692-01M

References & Citations

Van Rompay AR. et al. (1999) Mol. Pharmacol. 56:562-569.; ; Pearman AT. et al. (2001) Life Sci. 69:2361-2370.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_113620.1

Uniprot Accession Number

Q9HA47

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MFYSQEIRDM FHLRLFVDTD SDVRLSRRVL RDVRRGRDLE QILTQYTTFV KPAFEEFCLP TKKYADVIIP RGVDNMVAIN LIVQHIQDIL NGDICKWHRG GSNGRSYKRT FSEPGDHPGM LTSGKRSHLE SS