Recombinant human GDI1 protein

GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation. Recombinant human GDI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Rab GDP dissociation inhibitor alpha, Rab GDP dissociation inhibitor alpha, GDP dissociation inhibitor 1, 1A, GDIL, MRX41, MRX48, OPHN2, RABGD1A, RABGDIA, XAP-4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

53 kDa (470aa)

Additionnal Information

GDI1, Rab GDP dissociation inhibitor alpha, Rab GDP dissociation inhibitor alpha, GDP dissociation inhibitor 1, 1A, GDIL, MRX41, MRX48, OPHN2, RABGD1A, RABGDIA, XAP-4, ATGP2689-10 µg, ATGP2689-20 µg, ATGP2689-50 µg, ATGP2689-100 µg, ATGP2689-250 µg, ATGP2689-500 µg, ATGP2689-1 mg, ATGP2689-10, ATGP2689-20, ATGP2689-50, ATGP2689-100, ATGP2689-250, ATGP2689-500, ATGP2689-1

References & Citations

Bachner D, Sedlacek Z., et al. (1995) Hum Mol Genet 4 (4) : 701-8.; ; Sedlacek Z, Konecki DS., et al. (1995) Mamm Genome 5 (10) : 633-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001484.1

Uniprot Accession Number

P31150

Species

Human

AA Sequence

MDEEYDV IVLGTGLTEC ILSGIMSVNG KKVLHMDRNP YYGGESSSIT PLEELYKRFQ LLEGPPESMG RGRDWNVDLI PKFLMANGQL VKMLLYTEVT RYLDFKVVEG SFVYKGGKIY KVPSTETEAL ASNLMGMFEK RRFRKFLVFV ANFDENDPKT FEGVDPQTTS MRDVYRKFDL GQDVIDFTGH ALALYRTDDY LDQPCLETVN RIKLYSESLA RYGKSPYLYP LYGLGELPQG FARLSAIYGG TYMLNKPVDD IIMENGKVVG VKSEGEVARC KQLICDPSYI PDRVRKAGQV IRIICILSHP IKNTNDANSC QIIIPQNQVN RKSDIYVCMI SYAHNVAAQG KYIAIASTTV ETTDPEKEVE PALELLEPID QKFVAISDLY EPIDDGCESQ VFCSCSYDAT THFETTCNDI KDIYKRMAGT AFDFENMKRK QNDVFGEAEQ