Recombinant human PURB protein

PuRB is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PuR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. Recombinant human PuRB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Transcriptional activator protein Pur-beta, Purine-rich element binding protein B, PuRBETA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

35.6 kDa (335aa) confirmed by MALDI-TOF

Additionnal Information

PuRB, Transcriptional activator protein Pur-beta, Purine-rich element binding protein B, PuRBETA, ATGP2686-10 µg, ATGP2686-20 µg, ATGP2686-50 µg, ATGP2686-100 µg, ATGP2686-250 µg, ATGP2686-500 µg, ATGP2686-1 mg, ATGP2686-10, ATGP2686-20, ATGP2686-50, ATGP2686-100, ATGP2686-250, ATGP2686-500, ATGP2686-1

References & Citations

Bergemann A.D., Ma Z.-W., et al. (1992) Mol. Cell. Biol. 12:5673-5682

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_150093

Uniprot Accession Number

Q96QR8

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMADGDSG SERGGGGGPC GFQPASRGGG EQETQELASK RLDIQNKRFY LDVKQNAKGR FLKIAEVGAG GSKSRLTLSM AVAAEFRDSL GDFIEHYAQL GPSSPEQLAA GAEEGGGPRR ALKSEFLVRE NRKYYLDLKE NQRGRFLRIR QTVNRGGGGF GAGPGPGGLQ SGQTIALPAQ GLIEFRDALA KLIDDYGGED DELAGGPGGG AGGPGGGLYG ELPEGTSITV DSKRFFFDVG CNKYGVFLRV SEVKPSYRNA ITVPFKAWGK FGGAFCRYAD EMKEIQERQR DKLYERRGGG SGGGEESEGE EVDED