Recombinant human RDBP/NELFE protein

NELFE is a part of complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. It contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D) . Recombinant human NELFE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Negative elongation factor complex member E, RD RNA-binding protein, RNA-binding protein RD, RD, D6S45, NELF-E, RDP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by BRADFORD assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.6 kDa (403aa) confirmed by MALDI-TOF

Additionnal Information

NELFE, Negative elongation factor E, Negative elongation factor complex member E, D6S45, NELF-E, RD, RDBP, RDP, ATGP2685-10 µg, ATGP2685-20 µg, ATGP2685-50 µg, ATGP2685-100 µg, ATGP2685-250 µg, ATGP2685-500 µg, ATGP2685-1 mg, ATGP2685-10, ATGP2685-20, ATGP2685-50, ATGP2685-100, ATGP2685-250, ATGP2685-500, ATGP2685-1

References & Citations

Yamaguchi Y., Takagi T, et al. (1999) Cell 97:41-51; ; Surowy C.S., Hoganson G, et al. (1990) Gene 90:299-302;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 5mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002895

Uniprot Accession Number

P18615

Species

Human

AA Sequence

MLVIPPG LSEEEEALQK KFNKLKKKKK ALLALKKQSS SSTTSQGGVK RSLSEQPVMD TATATEQAKQ LVKSGAISAI KAETKNSGFK RSRTLEGKLK DPEKGPVPTF QPFQRSISAD DDLQESSRRP QRKSLYESFV SSSDRLRELG PDGEEAEGPG AGDGPPRSFD WGYEERSGAH SSASPPRSRS RDRSHERNRD RDRDRERDRD RDRDRDRERD RDRDRDRDRD RERDRDRERD RDRDREGPFR RSDSFPERRA PRKGNTLYVY GEDMTPTLLR GAFSPFGNII DLSMDPPRNC AFVTYEKMES ADQAVAELNG TQVESVQLKV NIARKQPMLD AATGKSVWGS LAVQNSPKGC HRDKRTQIVY SDDVYKENLV DGF