Recombinant human HLA-DOB protein

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Recombinant human HLA-DOB protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Major histocompatibility complex class II DO beta, Major histocompatibility complex, class II, DO beta, DOB

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.2 kDa (222aa)

Additionnal Information

HLA-DOB, Major histocompatibility complex class II DO beta, Major histocompatibility complex, class II, DO beta, DOB, ATGP2683-10 µg, ATGP2683-20 µg, ATGP2683-50 µg, ATGP2683-100 µg, ATGP2683-250 µg, ATGP2683-500 µg, ATGP2683-1 mg, ATGP2683-10, ATGP2683-20, ATGP2683-50, ATGP2683-100, ATGP2683-250, ATGP2683-500, ATGP2683-1

References & Citations

Naruse T.K., et al. (2002) Tissue Antigens. 59:512-519; ; Beck S., et al. (1996) J. Mol. Biol. 255:1-13;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002111

Uniprot Accession Number

P13765

Species

Human

AA Sequence

TDSPED FVIQAKADCY FTNGTEKVQF VVRFIFNLEE YVRFDSDVGM FVALTKLGQP DAEQWNSRLD LLERSRQAVD GVCRHNYRLG APFTVGRKVQ PEVTVYPERT PLLHQHNLLH CSVTGFYPGD IKIKWFLNGQ EERAGVMSTG PIRNGDWTFQ TVVMLEMTPE LGHVYTCLVD HSSLLSPVSV EWRAQSEYSW RK