Recombinant human PSG1 protein

PSG1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. PSGs are part of the carcinoembryonic antigen (CEA) family and serve as early biochemical markers of syncytiotrophoblast formation. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of PSG1 appears to regulate the affinity of DNA binding. Additionally, PSG1 is believed to mediate placental vascular morphogenesis by enhancing VEGF-A production and endothelial tube formation. Recombinant human PSG1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Pregnancy-specific beta-1-glycoprotein 1 isoform 1, Pregnancy-specific beta-1-glycoprotein 1 isoform 1, B1G1, CD66f, DHFRP2, FL-NCA-1/2, PBG1, PS-beta-C/D, PS-beta-G-1, PSBG-1, PSBG1, PSG95, PSGGA, PSGIIA, SP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

45.9 kDa (408aa)

Additionnal Information

PSG1, Pregnancy-specific beta-1-glycoprotein 1 isoform 1, Pregnancy-specific beta-1-glycoprotein 1 isoform 1, B1G1, CD66f, DHFRP2, FL-NCA-1/2, PBG1, PS-beta-C/D, PS-beta-G-1, PSBG-1, PSBG1, PSG95, PSGGA, PSGIIA, SP1, ATGP2682-10 µg, ATGP2682-20 µg, ATGP2682-50 µg, ATGP2682-100 µg, ATGP2682-250 µg, ATGP2682-500 µg, ATGP2682-1 mg, ATGP2682-10, ATGP2682-20, ATGP2682-50, ATGP2682-100, ATGP2682-250, ATGP2682-500, ATGP2682-1

References & Citations

Liu H., et al. (2002) Mol Cell Biol. 22: 6471-6479.; ; Van der Watt P J., et al. (2011) Biochim Biophys Acta. 1809: 316-326.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Other

NCBI Accession Number

NP_008836

Uniprot Accession Number

P11464

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSQVTIEAE PTKVSEGKDV LLLVHNLPQN LTGYIWYKGQ MRDLYHYITS YVVDGEIIIY GPAYSGRETA YSNASLLIQN VTREDAGSYT LHIIKGDDGT RGVTGRFTFT LHLETPKPSI SSSNLNPRET MEAVSLTCDP ETPDASYLWW MNGQSLPMTH SLKLSETNRT LFLLGVTKYT AGPYECEIRN PVSASRSDPV TLNLLPKLPK PYITINNLNP RENKDVLNFT CEPKSENYTY IWWLNGQSLP VSPRVKRPIE NRILILPSVT RNETGPYQCE IRDRYGGIRS DPVTLNVLYG PDLPRIYPSF TYYRSGEVLY LSCSADSNPP AQYSWTINEK FQLPGQKLFI RHITTKHSGL YVCSVRNSAT GKESSKSMTV EVSGKWIP