Recombinant human CAPNS1 protein

Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Recombinant human CAPNS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Calpain small subunit 1, Calpain, small subunit 1, 30K, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.8 kDa (208aa) confirmed by MALDI-TOF

Additionnal Information

CAPNS1, Calpain small subunit 1, Calpain, small subunit 1, 30K, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1, ATGP2681-10 µg, ATGP2681-20 µg, ATGP2681-50 µg, ATGP2681-100 µg, ATGP2681-250 µg, ATGP2681-500 µg, ATGP2681-1 mg, ATGP2681-10, ATGP2681-20, ATGP2681-50, ATGP2681-100, ATGP2681-250, ATGP2681-500, ATGP2681-1

References & Citations

Ohno S., Emori Y, et al. (1986) Nucleic Acids Res. 14:5559-5559

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_001740

Uniprot Accession Number

P04632

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG TGQIQVNIQE WLQLTMYS