Recombinant human CYB5R2 protein

Cytochrome b5 reductase 2, also known as CYB5R2, is involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. This protein is responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2, 4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1) . Recombinant human CYB5R2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cytochrome b5 reductase 2, B5R.2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

33.8 kDa (299aa) confirmed by MALDI-TOF

Additionnal Information

CYB5R2, Cytochrome b5 reductase 2, B5R.2, ATGP2679-10 µg, ATGP2679-20 µg, ATGP2679-50 µg, ATGP2679-100 µg, ATGP2679-250 µg, ATGP2679-500 µg, ATGP2679-1 mg, ATGP2679-10, ATGP2679-20, ATGP2679-50, ATGP2679-100, ATGP2679-250, ATGP2679-500, ATGP2679-1

References & Citations

Baker M.A., et al. (2005) Biol. Reprod. 73:334-342; ; Zhu H., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:14742-14747;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cardiovascular

NCBI Accession Number

NP_057313

Uniprot Accession Number

Q6BCY4

Species

Human

AA Sequence

MNSRRRE PITLQDPEAK YPLPLIEKEK ISHNTRRFRF GLPSPDHVLG LPVGNYVQLL AKIDNELVVR AYTPVSSDDD RGFVDLIIKI YFKNVHPQYP EGGKMTQYLE NMKIGETIFF RGPRGRLFYH GPGNLGIRPD QTSEPKKTLA DHLGMIAGGT GITPMLQLIR HITKDPSDRT RMSLIFANQT EEDILVRKEL EEIARTHPDQ FNLWYTLDRP PIGWKYSSGF VTADMIKEHL PPPAKSTLIL VCGPPPLIQT AAHPNLEKLG YTQDMIFTY