Recombinant human Selenoprotein H (SC44C) protein

SELH is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3' uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. The exact function of this gene is not known, however, selenoproteins are thought to be responsible for most biomedical effects of dietary selenium. Recombinant human SELH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Selenoprotein H, C17orf10, SELH, SELENOH

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

15.8 kDa (145aa) confirmed by MALDI-TOF

Additionnal Information

C17orf10, SELENOH, Selenoprotein H, SELH, SELH (SC44C), ATGP2674-10 µg, ATGP2674-20 µg, ATGP2674-50 µg, ATGP2674-100 µg, ATGP2674-250 µg, ATGP2674-500 µg, ATGP2674-1 mg, ATGP2674-010, ATGP2674-020, ATGP2674-050, ATGP2674-100, ATGP2674-250, ATGP2674-500, ATGP2674-01M

References & Citations

Panee J, Stoytcheva ZR, et al. (2007) . J Biol Chem. 282 (33) :23759-65. ; ; Novoselov SV, Kryukov GV, et al. (2007) . J Biol Chem. 282 (16) :11960-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Other

NCBI Accession Number

NP_734467

Uniprot Accession Number

Q8IZQ5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAPRGRK RKAEAAVVAV AEKREKLANG GEGMEEATVV IEHCTSCRVY GRNAAALSQA LRLEAPELPV KVNPTKPRRG SFEVTLLRPD GSSAELWTGI KKGPPRKLKF PEPQEVVEEL KKYLS