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Recombinant human CLPS protein

CLPS, also known as Colipase, is a protein co-enzyme required for optimal enzyme activity of pancreatic lipase. It is secreted by the pancreas in an inactive form, procolipase, which is activated in the intestinal lumen by trypsin. Its function is to prevent the inhibitory effect of bile salts on the lipase-catalyzed intraduodenal hydrolysis of dietary long-chain triglycerides. Recombinant human CLPS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Colipase isoform 1 preproprotein, Colipase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

12.5 kDa (118aa) confirmed by MALDI-TOF

Additionnal Information

CLPS, Colipase isoform 1 preproprotein, Colipase, ATGP2673-10 µg, ATGP2673-20 µg, ATGP2673-50 µg, ATGP2673-100 µg, ATGP2673-250 µg, ATGP2673-500 µg, ATGP2673-1 mg, ATGP2673-10, ATGP2673-20, ATGP2673-50, ATGP2673-100, ATGP2673-250, ATGP2673-500, ATGP2673-1

References & Citations

Lowe ME. et al. (1997) Annu. Rev. Nutr. 17:141-158.; ; Crandall WV. et al. (2001) J. Biol. Chem. 276:12505-12512;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 1mM DTT, 0.1M NaCl

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001823.1

Uniprot Accession Number

P04118

Species

Human

AA Sequence

APGPRGI IINLENGELC MNSAQCKSNC CQHSSALGLA RCTSMASENS ECSVKTLYGI YYKCPCERGL TCEGDKTIVG SITNTNFGIC HDAGRSKQ

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