Recombinant human CLPS protein
CLPS, also known as Colipase, is a protein co-enzyme required for optimal enzyme activity of pancreatic lipase. It is secreted by the pancreas in an inactive form, procolipase, which is activated in the intestinal lumen by trypsin. Its function is to prevent the inhibitory effect of bile salts on the lipase-catalyzed intraduodenal hydrolysis of dietary long-chain triglycerides. Recombinant human CLPS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Colipase isoform 1 preproprotein, Colipase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
12.5 kDa (118aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lowe ME. et al. (1997) Annu. Rev. Nutr. 17:141-158.; ; Crandall WV. et al. (2001) J. Biol. Chem. 276:12505-12512;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 1mM DTT, 0.1M NaCl
Scientific Category
Cardiovascular
NCBI Accession Number
NP_001823.1
Uniprot Accession Number
P04118
Species
Human
AA Sequence
APGPRGI IINLENGELC MNSAQCKSNC CQHSSALGLA RCTSMASENS ECSVKTLYGI YYKCPCERGL TCEGDKTIVG SITNTNFGIC HDAGRSKQ
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