Recombinant human BHMT2 protein

BHMT2 is involved in the regulation of homocysteine metabolism. Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. BHMT2 is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Recombinant human BHMT2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

S-methylmethionine-homocysteine S-methyltransferase BHMT2 isoform 1, betaine-homocysteine S-methyltransferase 2, SMM-hcy methyltransferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

42.7 kDa (386aa)

Additionnal Information

BHMT2, S-methylmethionine-homocysteine S-methyltransferase BHMT2 isoform 1, S-methylmethionine-homocysteine S-methyltransferase BHMT2 isoform 1, betaine--homocysteine S-methyltransferase 2, ATGP2672-10 µg, ATGP2672-20 µg, ATGP2672-50 µg, ATGP2672-100 µg, ATGP2672-250 µg, ATGP2672-500 µg, ATGP2672-1 mg, ATGP2672-10, ATGP2672-20, ATGP2672-50, ATGP2672-100, ATGP2672-250, ATGP2672-500, ATGP2672-1

References & Citations

Li F., et al. (2008) Mol Genet Metab. 94 (3) : 326-35.; ; Mostowaka A., et al. (2010) Eur Oral Sci. 118 (4) : 325-32.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_060084

Uniprot Accession Number

Q9H2M3

Species

Human

AA Sequence

MAPAGRP GAKKGILERL ESGEVVIGDG SFLITLEKRG YVKAGLWTPE AVIEHPDAVR QLHMEFLRAG SNVMQTFTFS ASEDNMESKW EDVNAAACDL AREVAGKGDA LVAGGICQTS IYKYQKDEAR IKKLFRQQLE VFAWKNVDFL IAEYFEHVEE AVWAVEVLKE SDRPVAVTMC IGPEGDMHDI TPGECAVRLV KAGASIVGVN CRFGPDTSLK TMELMKEGLE WAGLKAHLMV QPLGFHAPDC GKEGFVDLPE YPFGLESRVA TRWDIQKYAR EAYNLGVRYI GGCCGFEPYH IRAIAEELAP ERGFLPPASE KHGSWGSGLD MHTKPWIRAR ARREYWENLL PASGRPFCPS LSKPDF