Recombinant human HERC5 protein

HERC5 is a member of the HERC family of ubiquitin ligases. It is a protein with a HECT domain and five RCC1 repeats. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. HERC5 gene lies in a cluster of HERC family genes on chromosome 4. It has been shown to exhibit antiviral activity towards HIV-1, influenza A virus and human papillomavirus. Recombinant human HERC5 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

E3 ISG15-protein ligase HERC5, CEB1, CEBP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

43 kDa (367aa)

Additionnal Information

HERC5, E3 ISG15-protein ligase HERC5, CEB1, CEBP1, ATGP2671-10 µg, ATGP2671-20 µg, ATGP2671-50 µg, ATGP2671-100 µg, ATGP2671-250 µg, ATGP2671-500 µg, ATGP2671-1 mg, ATGP2671-10, ATGP2671-20, ATGP2671-50, ATGP2671-100, ATGP2671-250, ATGP2671-500, ATGP2671-1

References & Citations

Woods MW, Kelly JN, et al. (2011) . Retrovirology. 8:95. ; ; Tang Y, Zhong G, et al. (2010) . J Immunol. 184 (10) :5777-90.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_057407

Uniprot Accession Number

Q9UII4

Species

Human

AA Sequence

FDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG