Recombinant human ERO1L alpha protein

ERO1-like protein alpha, also known as EPO1L, is an essential oxidoreductase that oxidizes proteins and is required for the folding of immunoglobulins. EPO1L covalently binds with PDI (protein disulfide-isomerase) and together they produce disulfide bonds between proteins in the endoplasmic reticulum. EPO1L is stimulated by hypoxia, suggesting that it is regulated through the HIF (hypoxia inducible transcrip-tion factor) pathway. EPO1L is ubiquitously expressed at low levels but expressed at high levels in upper digestive tract and esophagus. Recombinant human EPO1L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ERO1-like protein alpha, ERO1-alpha, ERO1A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

54.4 kDa (468aa)

Additionnal Information

ERO1L a, ERO1-like protein alpha, ERO1-alpha, ERO1A, ATGP2669-10 µg, ATGP2669-20 µg, ATGP2669-50 µg, ATGP2669-100 µg, ATGP2669-250 µg, ATGP2669-500 µg, ATGP2669-1 mg, ATGP2669-10, ATGP2669-20, ATGP2669-50, ATGP2669-100, ATGP2669-250, ATGP2669-500, ATGP2669-1

References & Citations

Otsu M., et al. (2006) Antioxid. Redox Signal. 8: 274-282.; ; Gess B., et al. (2003) J Biochem. 270: 2228-2235.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_055399

Uniprot Accession Number

Q96HE7

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSEEQPPET AAQRCFCQVS GYLDDCTCDV ETIDRFNNYR LFPRLQKLLE SDYFRYYKVN LKRPCPFWND ISQCGRRDCA VKPCQSDEVP DGIKSASYKY SEEANNLIEE CEQAERLGAV DESLSEETQK AVLQWTKHDD SSDNFCEADD IQSPEAEYVD LLLNPERYTG YKGPDAWKIW NVIYEENCFK PQTIKRPLNP LASGQGTSEE NTFYSWLEGL CVEKRAFYRL ISGLHASINV HLSARYLLQE TWLEKKWGHN ITEFQQRFDG ILTEGEGPRR LKNLYFLYLI ELRALSKVLP FFERPDFQLF TGNKIQDEEN KMLLLEILHE IKSFPLHFDE NSFFAGDKKE AHKLKEDFRL HFRNISRIMD CVGCFKCRLW GKLQTQGLGT ALKILFSEKL IANMPESGPS YEFHLTRQEI VSLFNAFGRI STSVKELENF RNLLQNIH