Recombinant human ERO1L alpha protein
ERO1-like protein alpha, also known as EPO1L, is an essential oxidoreductase that oxidizes proteins and is required for the folding of immunoglobulins. EPO1L covalently binds with PDI (protein disulfide-isomerase) and together they produce disulfide bonds between proteins in the endoplasmic reticulum. EPO1L is stimulated by hypoxia, suggesting that it is regulated through the HIF (hypoxia inducible transcrip-tion factor) pathway. EPO1L is ubiquitously expressed at low levels but expressed at high levels in upper digestive tract and esophagus. Recombinant human EPO1L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
ERO1-like protein alpha, ERO1-alpha, ERO1A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
54.4 kDa (468aa)
Additionnal Information
References & Citations
Otsu M., et al. (2006) Antioxid. Redox Signal. 8: 274-282.; ; Gess B., et al. (2003) J Biochem. 270: 2228-2235.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_055399
Uniprot Accession Number
Q96HE7
Species
Human
AA Sequence
Frequently Asked Questions
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