Recombinant human REV1 protein

REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

DNA repair protein REV1, DNA repair protein REV1, REV1L

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

25.2 kDa (227aa)

Additionnal Information

REV1, DNA repair protein REV1, DNA repair protein REV1, REV1L, ATGP2668-10 µg, ATGP2668-20 µg, ATGP2668-50 µg, ATGP2668-100 µg, ATGP2668-250 µg, ATGP2668-500 µg, ATGP2668-1 mg, ATGP2668-10, ATGP2668-20, ATGP2668-50, ATGP2668-100, ATGP2668-250, ATGP2668-500, ATGP2668-1

References & Citations

Gibbs P.E.M., et al. (2000) Proc. Natl. Acad. Sci. u.S.A. 97:4186-4191; ; Masuda Y., et al. (2001) J. Biol. Chem. 276:15051-15058;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_057400

Uniprot Accession Number

Q9UBZ9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA