Recombinant human REV1 protein
REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
DNA repair protein REV1, DNA repair protein REV1, REV1L
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
25.2 kDa (227aa)
Additionnal Information
References & Citations
Gibbs P.E.M., et al. (2000) Proc. Natl. Acad. Sci. u.S.A. 97:4186-4191; ; Masuda Y., et al. (2001) J. Biol. Chem. 276:15051-15058;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_057400
Uniprot Accession Number
Q9UBZ9
Species
Human
AA Sequence
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