Recombinant human Coagulation Factor II/Thrombin protein

Coagulation factor II, also known as F2, is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Recombinant human F2 protein was expressed in E. coli.

Product Specifications

Product Name Alternative

Coagulation factor II, Factor II (thrombin), PT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

33.9 kDa (296aa)

Additionnal Information

Coagulation Factor II, Thrombin, Coagulation factor II, Factor II (thrombin), PT, ATGP2663-10 µg, ATGP2663-20 µg, ATGP2663-50 µg, ATGP2663-100 µg, ATGP2663-250 µg, ATGP2663-500 µg, ATGP2663-1 mg, ATGP2663-10, ATGP2663-20, ATGP2663-50, ATGP2663-100, ATGP2663-250, ATGP2663-500, ATGP2663-1

References & Citations

Royle NJ, Irwin DM, Koschinsky ML., et al. (1987) Somat. Cell Mol. Genet. 13 (3) : 285-92

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Apoptosis

NCBI Accession Number

NP_000497

Uniprot Accession Number

P00734

Species

Human

AA Sequence

MTFGSGEADC GLRPLFEKKS LEDKTERELL ESYIDGRIVE GSDAEIGMSP WQVMLFRKSP QELLCGASLI SDRWVLTAAH CLLYPPWDKN FTENDLLVRI GKHSRTRYER NIEKISMLEK IYIHPRYNWR ENLDRDIALM KLKKPVAFSD YIHPVCLPDR ETAASLLQAG YKGRVTGWGN LKETWTANVG KGQPSVLQVV NLPIVERPVC KDSTRIRITD NMFCAGYKPD EGKRGDACEG DSGGPFVMKS PFNNRWYQMG IVSWGEGCDR DGKYGFYTHV FRLKKWIQKV IDQFGE