Recombinant human PLA1A protein

PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene. Recombinant human PLA1A protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Phospholipase A1 member A, PS-PLA1, PSPLA1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

49.5 kDa (454aa)

Additionnal Information

PLA1A, Phospholipase A1 member A, PS-PLA1, PSPLA1, ATGP2661-10 µg, ATGP2661-20 µg, ATGP2661-50 µg, ATGP2661-100 µg, ATGP2661-250 µg, ATGP2661-500 µg, ATGP2661-1 mg, ATGP2661-10, ATGP2661-20, ATGP2661-50, ATGP2661-100, ATGP2661-250, ATGP2661-500, ATGP2661-1

References & Citations

Wang J., et al. (2002) J. Hum. Genet. 47:611-613; ; Nagai Y., et al. (1999) J. Biol. Chem. 274:11053-11059;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_056984

Uniprot Accession Number

Q53H76

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSDAPPTPQ PKCADFQSAN LFEGTDLKVQ FLLFVPSNPS CGQLVEGSSD LQNSGFNATL GTKLIIHGFR VLGTKPSWID TFIRTLLRAT NANVIAVDWI YGSTGVYFSA VKNVIKLSLE ISLFLNKLLV LGVSESSIHI IGVSLGAHVG GMVGQLFGGQ LGQITGLDPA GPEYTRASVE ERLDAGDALF VEAIHTDTDN LGIRIPVGHV DYFVNGGQDQ PGCPTFFYAG YSYLICDHMR AVHLYISALE NSCPLMAFPC ASYKAFLAGR CLDCFNPFLL SCPRIGLVEQ GGVKIEPLPK EVKVYLLTTS SAPYCMHHSL VEFHLKELRN KDTNIEVTFL SSNITSSSKI TIPKQQRYGK GIIAHATPQC QINQVKFKFQ SSNRVWKKDR TTIIGKFCTA LLPVNDREKM VCLPEPVNLQ ASVTVSCDLK IACV