Recombinant human GLUD1 protein
Glutamate dehydrogenase 1, mitochondrial precursor, also known as GLuD1, belongs to the Glu/Leu/Phe/Val dehydrogenases family. GLuD1 has a central role in nitrogen metabolism in plants and animals. GLuD1 is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Mutations in the gene encoding GLuD1 causes hyperinsulinism-hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood. GLuD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Recombinant human GLuD1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Glutamate dehydrogenase 1, mitochondrial, GDH, GDH1, GLUD
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
58.4 kDa (528aa)
Additionnal Information
References & Citations
Mastorodemos V., et al. (2009) Biochem Cell Biol. 87:505-516.; ; Tanizawa Y., et al. (2002) Diabetes. 51:712-717.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_005262
Uniprot Accession Number
P00367
Species
Human
AA Sequence
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