Recombinant human COPS7A protein
COPS7A is a component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JuN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JuN promotes and protects degradation by the ubl system, respectively. Recombinant human COPS7A protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
COP9 signalosome complex subunit 7a, COP9 signalosome complex subunit 7a, CSN7A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
32.7 kDa (298aa)
Additionnal Information
References & Citations
Wolf DA, Zhou C, et al. (2003) . Nat Cell Biol. 5 (12) :1029-33.; ; Wei N, Tsuge T, et al. (1998) . Curr Biol. 8 (16) :919-22.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Ubiquitination
NCBI Accession Number
NP_001157566
Uniprot Accession Number
Q9UBW8
Species
Human
AA Sequence
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