Recombinant human COPS7A protein

COPS7A is a component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JuN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JuN promotes and protects degradation by the ubl system, respectively. Recombinant human COPS7A protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

COP9 signalosome complex subunit 7a, COP9 signalosome complex subunit 7a, CSN7A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

32.7 kDa (298aa)

Additionnal Information

COPS7A, COP9 signalosome complex subunit 7a, COP9 signalosome complex subunit 7a, CSN7A, ATGP2652-10 µg, ATGP2652-20 µg, ATGP2652-50 µg, ATGP2652-100 µg, ATGP2652-250 µg, ATGP2652-500 µg, ATGP2652-1 mg, ATGP2652-10, ATGP2652-20, ATGP2652-50, ATGP2652-100, ATGP2652-250, ATGP2652-500, ATGP2652-1

References & Citations

Wolf DA, Zhou C, et al. (2003) . Nat Cell Biol. 5 (12) :1029-33.; ; Wei N, Tsuge T, et al. (1998) . Curr Biol. 8 (16) :919-22.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_001157566

Uniprot Accession Number

Q9UBW8

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSAEVKV TGQNQEQFLL LAKSAKGAAL ATLIHQVLEA PGVYVFGELL DMPNVRELAE SDFASTFRLL TVFAYGTYAD YLAEARNLPP LTEAQKNKLR HLSVVTLAAK VKCIPYAVLL EALALRNVRQ LEDLVIEAVY ADVLRGSLDQ RNQRLEVDYS IGRDIQRQDL SAIARTLQEW CVGCEVVLSG IEEQVSRANQ HKEQQLGLKQ QIESEVANLK KTIKVTTAAA AAATSQDPEQ HLTELREPAP GTNQRQPSKK ASKGKGLRGS AKIWSKSN