Recombinant human MED27 protein
MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e. g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Recombinant human MED27 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Mediator complex subunit 27, CRAP34, CRSP34, CRSP8, TRAP37
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
37.8 kDa (334aa)
Additionnal Information
References & Citations
Malik S., et al. (2000) Mol. Cell. 5:753-760; ; Ryu S., et al. (1999) Nature. 397:446-450;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_004260.2
Uniprot Accession Number
Q6P2C8
Species
Human
AA Sequence
Frequently Asked Questions
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