Recombinant human MED27 protein

MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e. g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Recombinant human MED27 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Mediator complex subunit 27, CRAP34, CRSP34, CRSP8, TRAP37

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

37.8 kDa (334aa)

Additionnal Information

MED27, Mediator complex subunit 27, CRAP34, CRSP34, CRSP8, TRAP37, ATGP2650-10 µg, ATGP2650-20 µg, ATGP2650-50 µg, ATGP2650-100 µg, ATGP2650-250 µg, ATGP2650-500 µg, ATGP2650-1 mg, ATGP2650-10, ATGP2650-20, ATGP2650-50, ATGP2650-100, ATGP2650-250, ATGP2650-500, ATGP2650-1

References & Citations

Malik S., et al. (2000) Mol. Cell. 5:753-760; ; Ryu S., et al. (1999) Nature. 397:446-450;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004260.2

Uniprot Accession Number

Q6P2C8

Species

Human

AA Sequence

MADVINV SVNLEAFSQA ISAIQALRSS VSRVFDCLKD GMRNKETLEG REKAFIAHFQ DNLHSVNRDL NELERLSNLV GKPSENHPLH NSGLLSLDPV QDKTPLYSQL LQAYKWSNKL QYHAGLASGL LNQQSLKRSA NQMGVSAKRR PKAQPTTLVL PPQYVDDVIS RIDRMFPEMS IHLSRPNGTS AMLLVTLGKV LKVIVVMRSL FIDRTIVKGY NENVYTEDGK LDIWSKSNYQ VFQKVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ