Recombinant human RNA polymerases I, II, and III subunit RPABC2/POLR2F protein

POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA-directed RNA polymerases I II and III subunit RPABC2, DNA-directed RNA polymerases I, II, and III subunit RPABC2, Polymerase (RNA) II (DNA directed) polypeptide F, HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BRADFORD assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.9 kDa (150aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

POLR2F, DNA-directed RNA polymerases I II and III subunit RPABC2, DNA-directed RNA polymerases I, II, and III subunit RPABC2, Polymerase (RNA) II (DNA directed) polypeptide F, HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6, ATGP2647-10 µg, ATGP2647-20 µg, ATGP2647-50 µg, ATGP2647-100 µg, ATGP2647-250 µg, ATGP2647-500 µg, ATGP2647-1 mg, ATGP2647-10, ATGP2647-20, ATGP2647-50, ATGP2647-100, ATGP2647-250, ATGP2647-500, ATGP2647-1

References & Citations

Kayukawa K., Makino Y., et al. (1999) Gene 234:139-147

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_068809

Uniprot Accession Number

P61218

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD