Recombinant human RNA polymerases I, II, and III subunit RPABC2/POLR2F protein
POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BRADFORD assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
16.9 kDa (150aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Kayukawa K., Makino Y., et al. (1999) Gene 234:139-147
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_068809
Uniprot Accession Number
P61218
Species
Human
AA Sequence
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