Recombinant human DNMT3L protein

DNA (cytosine-5) -methyltransferase 3-like isoform, also known as DNMT3L, is a 387 amino acid protein that contains one ADD-type zinc finger and is a member of the DNMT family. Localized to the nucleus and expressed at lows levels in thymus, testis and ovary, DNMT3L does not exhibit DNA methyltransferase activity, but is able to stimulate de novo methylation by DNMT3 and is thought to play a key role in the establishment of genomic imprints. Recombinant human DNMT3L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA (cytosine-5) -methyltransferase 3-like isoform, DNA (cytosine-5) -methyltransferase 3-like isoform 2, DNA (cytosine-5-) -methyltransferase 3-like

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

46.3 kDa (412aa) confirmed by MALDI-TOF

Additionnal Information

DNMT3L, DNA (cytosine-5) -methyltransferase 3-like isoform, DNA (cytosine-5) -methyltransferase 3-like isoform 2, DNA (cytosine-5-) -methyltransferase 3-like, ATGP2634-10 µg, ATGP2634-20 µg, ATGP2634-50 µg, ATGP2634-100 µg, ATGP2634-250 µg, ATGP2634-500 µg, ATGP2634-1 mg, ATGP2634-10, ATGP2634-20, ATGP2634-50, ATGP2634-100, ATGP2634-250, ATGP2634-500, ATGP2634-1

References & Citations

Yoder J A., et al. (1997) J Mol Biol. 270: 385-395.; ; Suetake I., et al. (2006) J Biochem 1405: 553-559.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_037501

Uniprot Accession Number

Q9UJW3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSEFMAAIP ALDPEAEPSM DVILVGSSEL SSSVSPGTGR DLIAYEVKAN QRNIEDICIC CGSLQVHTQH PLFEGGICAP CKDKFLDALF LYDDDGYQSY CSICCSGETL LICGNPDCTR CYCFECVDSL VGPGTSGKVH AMSNWVCYLC LPSSRSGLLQ RRRKWRSQLK AFYDRESENP LEMFETVPVW RRQPVRVLSL FEDIKKELTS LGFLESGSDP GQLKHVVDVT DTVRKDVEEW GPFDLVYGAT PPLGHTCDRP PSWYLFQFHR LLQYARPKPG SPRPFFWMFV DNLVLNKEDL DVASRFLEME PVTIPDVHGG SLQNAVRVWS NIPAIRSSRH WALVSEEELS LLAQNKQSSK LAAKWPTKLV KNCFLPLREY FKYFSTELTS SL