Recombinant human DNMT3L protein
DNA (cytosine-5) -methyltransferase 3-like isoform, also known as DNMT3L, is a 387 amino acid protein that contains one ADD-type zinc finger and is a member of the DNMT family. Localized to the nucleus and expressed at lows levels in thymus, testis and ovary, DNMT3L does not exhibit DNA methyltransferase activity, but is able to stimulate de novo methylation by DNMT3 and is thought to play a key role in the establishment of genomic imprints. Recombinant human DNMT3L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA (cytosine-5) -methyltransferase 3-like isoform, DNA (cytosine-5) -methyltransferase 3-like isoform 2, DNA (cytosine-5-) -methyltransferase 3-like
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
46.3 kDa (412aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Yoder J A., et al. (1997) J Mol Biol. 270: 385-395.; ; Suetake I., et al. (2006) J Biochem 1405: 553-559.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_037501
Uniprot Accession Number
Q9UJW3
Species
Human
AA Sequence
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