Recombinant human EXOSC8 protein

EXOSC8, also known as exosome component 8 and transcription factor 4, is a part of the exosome complex. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing Au-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC8 binds to ARE-containing RNAs. Recombinant human EXOSC8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Exosome complex component RRP43, bA421P11.3, CIP3, EAP2, OIP2, p9, RP11- 421P11.3, RRP43, Rrp43p

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.4 kDa (299aa) confirmed by MALDI-TOF

Additionnal Information

EXOSC8, Exosome complex component RRP43, bA421P11.3, CIP3, EAP2, OIP2, p9, RP11- 421P11.3, RRP43, Rrp43p, ATGP2629-10 µg, ATGP2629-20 µg, ATGP2629-50 µg, ATGP2629-100 µg, ATGP2629-250 µg, ATGP2629-500 µg, ATGP2629-1 mg, ATGP2629-10, ATGP2629-20, ATGP2629-50, ATGP2629-100, ATGP2629-250, ATGP2629-500, ATGP2629-1

References & Citations

Andersen JS. et al. (2005) Nature. 433:77-83; ; Gerhard DS. et al. (2004) Gerhard DS. 14 : 2121-2127.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol 0.2M NaCl,1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_852480

Uniprot Accession Number

Q96B26

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAAGFKT VEPLEYYRRF LKENCRPDGR ELGEFRTTTV NIGSISTADG SALVKLGNTT VICGVKAEFA APSTDAPDKG YVVPNVDLPP LCSSRFRSGP PGEEAQVASQ FIADVIENSQ IIQKEDLCIS PGKLVWVLYC DLICLDYDGN ILDACTFALL AALKNVQLPE VTINEETALA EVNLKKKSYL NIRTHPVATS FAVFDDTLLI VDPTGEEEHL ATGTLTIVMD EEGKLCCLHK PGGSGLTGAK LQDCMSRAVT RHKEVKKLMD EVIKSMKPK