Recombinant human PAICS protein

PAICS, also known as phosphoribosylaminoimidazole carboxylase, is an enzyme involved in nucleotide biosynthesis and in particular in purine biosynthesis. It catalyzes the conversion of 5'-phosphoribosyl-5-aminoimidazole (AIR) into 5'-phosphoribosyl-4-carboxy-5-aminoimidazole (CAIR) as described in the reaction. Recombinant human PAICS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Multifunctional protein ADE2, ADE2, ADE2H1, AIRC, PAIS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

49.5 kDa (448aa) confirmed by MALDI-TOF

Additionnal Information

PAICS, Multifunctional protein ADE2, ADE2, ADE2H1, AIRC, PAIS, ATGP2628-10 µg, ATGP2628-20 µg, ATGP2628-50 µg, ATGP2628-100 µg, ATGP2628-250 µg, ATGP2628-500 µg, ATGP2628-1 mg, ATGP2628-10, ATGP2628-20, ATGP2628-50, ATGP2628-100, ATGP2628-250, ATGP2628-500, ATGP2628-1

References & Citations

Ealick SE. et al. (1999) Structure. 7:1395-1406; ; Meyer E. et al. (1999) Biochemistry. 38:3012-3018.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001072992

Uniprot Accession Number

P22234

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMATAEVL NIGKKLYEGK TKEVYELLDS PGKVLLQSKD QITAGNAARK NHLEGKAAIS NKITSCIFQL LQEAGIKTAF TRKCGETAFI APQCEMIPIE WVCRRIATGS FLKRNPGVKE GYKFYPPKVE LFFKDDANND PQWSEEQLIA AKFCFAGLLI GQTEVDIMSH ATQAIFEILE KSWLPQNCTL VDMKIEFGVD VTTKEIVLAD VIDNDSWRLW PSGDRSQQKD KQSYRDLKEV TPEGLQMVKK NFEWVAERVE LLLKSESQCR VVVLMGSTSD LGHCEKIKKA CGNFGIPCEL RVTSAHKGPD ETLRIKAEYE GDGIPTVFVA VAGRSNGLGP VMSGNTAYPV ISCPPLTPDW GVQDVWSSLR LPSGLGCSTV LSPEGSAQFA AQIFGLSNHL VWSKLRASIL NTWISLKQAD KKIRECNL