Recombinant human PAICS protein
PAICS, also known as phosphoribosylaminoimidazole carboxylase, is an enzyme involved in nucleotide biosynthesis and in particular in purine biosynthesis. It catalyzes the conversion of 5'-phosphoribosyl-5-aminoimidazole (AIR) into 5'-phosphoribosyl-4-carboxy-5-aminoimidazole (CAIR) as described in the reaction. Recombinant human PAICS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Multifunctional protein ADE2, ADE2, ADE2H1, AIRC, PAIS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
49.5 kDa (448aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ealick SE. et al. (1999) Structure. 7:1395-1406; ; Meyer E. et al. (1999) Biochemistry. 38:3012-3018.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001072992
Uniprot Accession Number
P22234
Species
Human
AA Sequence
Frequently Asked Questions
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