Recombinant human Glypican 4 protein
Glypican 4, also known as GPC4, is a family of glycosylphosphatidylinositol (GPI) -anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. GPC4 is widely expressed in human tissues, including lung, kidney, heart, placenta, skeletal muscle, and pancreas. GPC4 has also been shown to be present in astrocytes, haematopoietic-progenitor and bone-marrow-stromal cells. Recombinant human GPC4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glypican 4, Glypican 4, dJ900E8.1, glypican proteoglycan 4
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
18 kDa (165aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Fico A., et al. (2012) Stem Cells. 30 (9) : 1863-74.; ; Ybot Gonzalez P., et al. (2005) Dev Dyn. 233 (3) : 1013-7.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
Scientific Category
Stem cell
NCBI Accession Number
NP_001439.2
Uniprot Accession Number
O75487
Species
Human
AA Sequence
SSLP SNVCNDERMA AGNGNEDDCW NGKGKSRYLF AVTGNGLANQ GNNPEVQVDT SKPDILILRQ IMALRVMTSK MKNAYNGNDV DFFDISDESS GEGSGSGCEY QQCPSEFDYN ATDHAGKSAN EKADS
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