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Recombinant human Glypican 4 protein

Glypican 4, also known as GPC4, is a family of glycosylphosphatidylinositol (GPI) -anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. GPC4 is widely expressed in human tissues, including lung, kidney, heart, placenta, skeletal muscle, and pancreas. GPC4 has also been shown to be present in astrocytes, haematopoietic-progenitor and bone-marrow-stromal cells. Recombinant human GPC4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glypican 4, Glypican 4, dJ900E8.1, glypican proteoglycan 4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

18 kDa (165aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Glypican4, Glypican 4, Glypican 4, dJ900E8.1, glypican proteoglycan 4, ATGP2616-10 µg, ATGP2616-20 µg, ATGP2616-50 µg, ATGP2616-100 µg, ATGP2616-250 µg, ATGP2616-500 µg, ATGP2616-1 mg, ATGP2616-10, ATGP2616-20, ATGP2616-50, ATGP2616-100, ATGP2616-250, ATGP2616-500, ATGP2616-1

References & Citations

Fico A., et al. (2012) Stem Cells. 30 (9) : 1863-74.; ; Ybot Gonzalez P., et al. (2005) Dev Dyn. 233 (3) : 1013-7.;

Other References

Nakashima Y, et al. Atorvastatin Inhibits the HIF1α-PPAR Axis, Which Is Essential for Maintaining the Function of Human Induced Pluripotent Stem Cells. (Mol Ther. 2018) {https://pubmed.ncbi.nlm.nih.gov/29929789/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Stem cell

NCBI Accession Number

NP_001439.2

Uniprot Accession Number

O75487

Species

Human

AA Sequence

SSLP SNVCNDERMA AGNGNEDDCW NGKGKSRYLF AVTGNGLANQ GNNPEVQVDT SKPDILILRQ IMALRVMTSK MKNAYNGNDV DFFDISDESS GEGSGSGCEY QQCPSEFDYN ATDHAGKSAN EKADS

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