Recombinant human CD40/TNFRSF5 protein
CD40 is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Recombinant human CD40 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, p50, CD40L receptor, CDw40
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
21.6 kDa (196aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Tone M., et al. (2001) Proc. Natl. Acad. Sci. u.S.A. 98:1751-1756; ; Khandekar S.S., et al. (2001) Protein Expr. Purif. 23:301-310;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Stem cell
NCBI Accession Number
NP_001241.1
Uniprot Accession Number
P25942
Species
Human
AA Sequence
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