Recombinant human MGAT2 protein

MGAT2 is a golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Transcript variants with a spliced 5' uTR may exist, but their biological validity has not been determined. Recombinant human MGAT2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Mannosyl alpha-1,6-glycoprotein beta-1,2-N-acetylglucosaminyltransferas, GlcNAc-T II, GNT-II, Mannoside acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, CDG2A, CDGS2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

50 kDa (439aa)

Additionnal Information

MGAT2, Mannosyl (alpha-16-) -glycoprotein beta-12-N-acetylglucosaminyltransferase, Mannosyl (alpha-1,6-) -glycoprotein beta-1,2-N-acetylglucosaminyltransferase, CDGS2, GLCNACTII, GNT-II, GNT2, ATGP2614-10 µg, ATGP2614-20 µg, ATGP2614-50 µg, ATGP2614-100 µg, ATGP2614-250 µg, ATGP2614-500 µg, ATGP2614-1 mg, ATGP2614-10, ATGP2614-20, ATGP2614-50, ATGP2614-100, ATGP2614-250, ATGP2614-500, ATGP2614-1

References & Citations

Tan J, Dunn J, et al. (1996) . Am J Hum Genet. 59 (4) :810-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_002399

Uniprot Accession Number

Q10469

Species

Human

AA Sequence

MRQRKNEALA PPLLDAEPAR GAGGRGGDHP SVAVGIRRVS NVSAASLVPA VPQPEADNLT LRYRSLVYQL NFDQTLRNVD KAGTWAPREL VLVVQVHNRP EYLRLLLDSL RKAQGIDNVL VIFSHDFWST EINQLIAGVN FCPVLQVFFP FSIQLYPNEF PGSDPRDCPR DLPKNAALKL GCINAEYPDS FGHYREAKFS QTKHHWWWKL HFVWERVKIL RDYAGLILFL EEDHYLAPDF YHVFKKMWKL KQQECPECDV LSLGTYSASR SFYGMADKVD VKTWKSTEHN MGLALTRNAY QKLIECTDTF CTYDDYNWDW TLQYLTVSCL PKFWKVLVPQ IPRIFHAGDC GMHHKKTCRP STQSAQIESL LNNNKQYMFP ETLTISEKFT VVAISPPRKN GGWGDIRDHE LCKSYRRLQ