Recombinant human CD1e protein

CD1E is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. CD1E localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members. Recombinant human CD1E protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

T-cell surface glycoprotein CD1e, T-cell surface glycoprotein CD1e, CD1A, R2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

33.1 kDa (297aa)

Additionnal Information

CD1e, T-cell surface glycoprotein CD1e, T-cell surface glycoprotein CD1e, CD1A, R2, ATGP2609-10 µg, ATGP2609-20 µg, ATGP2609-50 µg, ATGP2609-100 µg, ATGP2609-250 µg, ATGP2609-500 µg, ATGP2609-1 mg, ATGP2609-10, ATGP2609-20, ATGP2609-50, ATGP2609-100, ATGP2609-250, ATGP2609-500, ATGP2609-1

References & Citations

Angenieux C., et al (2000) . J. Biol. Chem. 275:37757-37764; ; de la Salle H., et al (2005) . Science 310:1321-1324;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Immunology

NCBI Accession Number

NP_112155

Uniprot Accession Number

P15812

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSEEQLSFR MLQTSSFANH SWAHSEGSGW LGDLQTHGWD TVLGTIRFLK PWSHGNFSKQ ELKNLQSLFQ LYFHSFIQIV QASAGQFQLE YPFEIQILAG CRMNAPQIFL NMAYQGSDFL SFQGISWEPS PGAGIRAQNI CKVLNRYLDI KEILQSLLGH TCPRFLAGLM EAGESELKRK VKPEAWLSCG PSPGPGRLQL VCHVSGFYPK PVWVMWMRGE QEQRGTQRGD VLPNADETWY LRATLDVAAG EAAGLSCRVK HSSLGGHDLI IHWGGYS