Recombinant human TCF4 protein

TCF4, also known as immunoglobulin transcription factor 2 and transcription factor 4, is a protein acting as a transcription factor. This protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. It is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Recombinant human TCF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Transcription factor 4, bHLHb19, E2-2, ITF-2, ITF2, PTHS, SEF-2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, TCF-4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

56.6 kDa (530aa)

Additionnal Information

TCF4, Transcription factor 4, bHLHb19, E2-2, ITF-2, ITF2, PTHS, SEF-2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, TCF-4, ATGP2605-10 µg, ATGP2605-20 µg, ATGP2605-50 µg, ATGP2605-100 µg, ATGP2605-250 µg, ATGP2605-500 µg, ATGP2605-1 mg, ATGP2605-10, ATGP2605-20, ATGP2605-50, ATGP2605-100, ATGP2605-250, ATGP2605-500, ATGP2605-1

References & Citations

Sepp, M. et al. (2012) Human Molecular Genetics. 21:2873-2888; ; de Pontual L. et al. (2009) Hum. Mutat. 30:669-676.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Stem cell

NCBI Accession Number

NP_001230165.1

Uniprot Accession Number

P15884

Species

Human

AA Sequence

MKFKQCR CSDTGLCCLD HEGKAEVYAP SASTADYNRD SPGYPSSKPA TSTFPSSFFM QDGHHSSDPW SSSSGMNQPG YAGMLGNSSH IPQSSSYCSL HPHERLSYPS HSSADINSSL PPMSTFHRSG TNHYSTSSCT PPANGTDSIM ANRGSGAAGS SQTGDALGKA LASIYSPDHT NNSFSSNPST PVGSPPSLSA GTAVWSRNGG QASSSPNYEG PLHSLQSRIE DRLERLDDAI HVLRNHAVGP STAMPGGHGD MHGIIGPSHN GAMGGLGSGY GTGLLSANRH SLMVGTHRED GVALRGSHSL LPNQVPVPQL PVQSATSPDL NPPQDPYRGM PPGLQGQSVS SGSSEIKSDD EGDENLQDTK SSEDKKLDDD KKDIKSITSN NDDEDLTPEQ KAEREKERRM ANNARERLRV RDINEAFKEL GRMVQLHLKS DKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSSEPPP LSLAGPHPGM GDASNHMGQM