Recombinant human BEST1 protein
BEST1 is a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Recombinant human BEST1 protein, fused to His-tag at N-terminus, was expressed in E. coli
Product Specifications
Product Name Alternative
Bestrophin-1 isoform 1, ARB, BEST, BMD, RP50, Tu15B, VMD2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36 kDa (317aa)
Additionnal Information
References & Citations
Sodi A, Passerini I, et al. (2012) . Mol Vis.18:2736-48.; ; Bitner H, Schatz P, et al. (2012) . Am J Ophthalmol. 154 (2) :403-412.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_004174.1
Uniprot Accession Number
O76090
Species
Human
AA Sequence
Frequently Asked Questions
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