Recombinant human BEST1 protein

BEST1 is a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Recombinant human BEST1 protein, fused to His-tag at N-terminus, was expressed in E. coli

Product Specifications

Product Name Alternative

Bestrophin-1 isoform 1, ARB, BEST, BMD, RP50, Tu15B, VMD2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36 kDa (317aa)

Additionnal Information

BEST1, Bestrophin-1 isoform 1, ARB, BEST, BMD, RP50, Tu15B, VMD2, ATGP2600-10 µg, ATGP2600-20 µg, ATGP2600-50 µg, ATGP2600-100 µg, ATGP2600-250 µg, ATGP2600-500 µg, ATGP2600-1 mg, ATGP2600-10, ATGP2600-20, ATGP2600-50, ATGP2600-100, ATGP2600-250, ATGP2600-500, ATGP2600-1

References & Citations

Sodi A, Passerini I, et al. (2012) . Mol Vis.18:2736-48.; ; Bitner H, Schatz P, et al. (2012) . Am J Ophthalmol. 154 (2) :403-412.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_004174.1

Uniprot Accession Number

O76090

Species

Human

AA Sequence

EQLINPF GEDDDDFETN WIVDRNLQVS LLAVDEMHQD LPRMEPDMYW NKPEPQPPYT AASAQFRRAS FMGSTFNISL NKEEMEFQPN QEDEEDAHAG IIGRFLGLQS HDHHPPRANS RTKLLWPKRE SLLHEGLPKN HKAAKQNVRG QEDNKAWKLK AVDAFKSAPL YQRPGYYSAP QTPLSPTPMF FPLEPSAPSK LHSVTGIDTK DKSLKTVSSG AKKSFELLSE SDGALMEHPE VSQVRRKTVE FNLTDMPEIP ENHLKEPLEQ SPTNIHTTLK DHMDPYWALE NRDEAHS