Recombinant human KRT16 protein

KRT16 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. Recombinant human KRT16 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Keratin type I cytoskeletal 16, Keratin, type I cytoskeletal 16, CK16, FNEPPK, K16, K1CP, KRT16A, NEPPK

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

53.7 kDa (496aa)

Additionnal Information

KRT16, Keratin type I cytoskeletal 16, Keratin, type I cytoskeletal 16, CK16, FNEPPK, K16, K1CP, KRT16A, NEPPK, ATGP2599-10 µg, ATGP2599-20 µg, ATGP2599-50 µg, ATGP2599-100 µg, ATGP2599-250 µg, ATGP2599-500 µg, ATGP2599-1 mg, ATGP2599-10, ATGP2599-20, ATGP2599-50, ATGP2599-100, ATGP2599-250, ATGP2599-500, ATGP2599-1

References & Citations

Du ZF, Xu CM, Zhao Y, et al. (2012) . Eur J Dermatol. 22 (4) :476-80.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005548

Uniprot Accession Number

P08779

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMTTCSRQ FTSSSSMKGS CGIGGGIGGG SSRISSVLAG GSCRAPSTYG GGLSVSSRFS SGGACGLGGG YGGGFSSSSS FGSGFGGGYG GGLGAGFGGG LGAGFGGGFA GGDGLLVGSE KVTMQNLNDR LASYLDKVRA LEEANADLEV KIRDWYQRQR PSEIKDYSPY FKTIEDLRNK IIAATIENAQ PILQIDNARL AADDFRTKYE HELALRQTVE ADVNGLRRVL DELTLARTDL EMQIEGLKEE LAYLRKNHEE EMLALRGQTG GDVNVEMDAA PGVDLSRILN EMRDQYEQMA EKNRRDAETW FLSKTEELNK EVASNSELVQ SSRSEVTELR RVLQGLEIEL QSQLSMKASL ENSLEETKGR YCMQLSQIQG LIGSVEEQLA QLRCEMEQQS QEYQILLDVK TRLEQEIATY RRLLEGEDAH LSSQQASGQS YSSREVFTSS SSSSSRQTRP ILKEQSSSSF SQGQSS