Recombinant human SKA1 protein
SKA1, as known as spindle and kinetochore-associated protein 1, is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Recombinant human SKA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Spindle and kinetochore-associated protein 1, Spindle and kinetochore-associated protein 1, C18orf24
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
31.9 kDa (278aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ota T. et al. (2004) Nat Genet. 36:40-45; ; Hanisch A. et al. (2006) EMBO J. 25: 5504-5515.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Scientific Category
Cell Cycle
NCBI Accession Number
NP_001034624
Uniprot Accession Number
Q96BD8
Species
Human
AA Sequence
Frequently Asked Questions
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