Recombinant human SKA1 protein

SKA1, as known as spindle and kinetochore-associated protein 1, is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Recombinant human SKA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Spindle and kinetochore-associated protein 1, Spindle and kinetochore-associated protein 1, C18orf24

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

31.9 kDa (278aa) confirmed by MALDI-TOF

Additionnal Information

SKA1, Spindle and kinetochore-associated protein 1, Spindle and kinetochore-associated protein 1, C18orf24, ATGP2596-10 µg, ATGP2596-20 µg, ATGP2596-50 µg, ATGP2596-100 µg, ATGP2596-250 µg, ATGP2596-500 µg, ATGP2596-1 mg, ATGP2596-10, ATGP2596-20, ATGP2596-50, ATGP2596-100, ATGP2596-250, ATGP2596-500, ATGP2596-1

References & Citations

Ota T. et al. (2004) Nat Genet. 36:40-45; ; Hanisch A. et al. (2006) EMBO J. 25: 5504-5515.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_001034624

Uniprot Accession Number

Q96BD8

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMASSDLE QLCSHVNEKI GNIKKTLSLR NCGQEPTLKT VLNKIGDEII VINELLNKLE LEIQYQEQTN NSLKELCESL EEDYKDIEHL KENVPSHLPQ VTVTQSCVKG SDLDPEEPIK VEEPEPVKKP PKEQRSIKEM PFITCDEFNG VPSYMKSRLT YNQINDVIKE INKAVISKYK ILHQPKKSMN SVTRNLYHRF IDEETKDTKG RYFIVEADIK EFTTLKADKK FHVLLNILRH CRRLSEVRGG GLTRYVIT