Recombinant human IYD protein

IYD is an enzyme that catalyzes the oxidative NADPH-dependent deiodination of mono- and diiodotyrosine, which are the halogenated byproducts of thyroid hormone production. The N-terminus of the protein functions as a membrane anchor. Mutations in this gene cause congenital hypothyroidism due to dyshormonogenesis type 4, which is also referred to as deiodinase deficiency, or iodotyrosine dehalogenase deficiency, or thyroid hormonogenesis type 4. Alternative splicing results in multiple transcript variants. Recombinant human IYD protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Iodotyrosine dehalogenase 1 isoform 3, C6orf71, DEHAL1, dJ422F24.1, TDH4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.1 kDa (214aa)

Additionnal Information

IYD, iodotyrosine dehalogenase 1 isoform 3, C6orf71, DEHAL1, dJ422F24.1, TDH4, ATGP2592-10 µg, ATGP2592-20 µg, ATGP2592-50 µg, ATGP2592-100 µg, ATGP2592-250 µg, ATGP2592-500 µg, ATGP2592-1 mg, ATGP2592-10, ATGP2592-20, ATGP2592-50, ATGP2592-100, ATGP2592-250, ATGP2592-500, ATGP2592-1

References & Citations

Burniat, A, et al. (2012) J. Clin. Endocrinol. Metab. 97 (7), E1276-E1283; ; Belevich, V.K., et al. (2011) Biomed Khim 57 (2), 187-194;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001158167

Uniprot Accession Number

Q6PHW0

Species

Human

AA Sequence

DRSMEKK KGEPRTRAEA RPWVDEDLKD SSDLHQAEED ADEWQESEEN VEHIPFSHNH YPEKEMVKRS QEFYELLNKR RSVRFISNEQ VPMEVIDNVI RTAGTAPSGA HTEPWTFVVV KDPDVKHKIR KIIEEEEEIN YMKRMGHRWV TDLKKLRTNW IKEYLDTAPI LILIFKQVHG FAANGKKKVH YYNE