Recombinant human SOCS-3 protein

SOCS3 is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of SOCS3 is induced by various cytokines, including IL6, IL10, and interferon (IFN) -gamma. This protein can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. Recombinant human SOCS3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Suppressor of cytokine signaling 3, CIS3, SSI3, ATOD4, Cish3, SSI-3, SOCS-3, MGC71791

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29 kDa (262aa)

Additionnal Information

SOCS3, Suppressor of cytokine signaling 3, CIS3, SSI3, ATOD4, Cish3, SSI-3, SOCS-3, MGC71791, ATGP2589-10 µg, ATGP2589-20 µg, ATGP2589-50 µg, ATGP2589-100 µg, ATGP2589-250 µg, ATGP2589-500 µg, ATGP2589-1 mg, ATGP2589-10, ATGP2589-20, ATGP2589-50, ATGP2589-100, ATGP2589-250, ATGP2589-500, ATGP2589-1

References & Citations

McKay BR, Ogborn DI, et al. (2013) . Am J Physiol Cell Physiol. 304 (8) :C717-28.; ; Molavi O, Wang P, et al. (2013) . Br J Haematol. 161 (3) :348-56.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_003946

Uniprot Accession Number

O14543

Species

Human

AA Sequence

MGRDLYDDDD KDRWGSHMVT HSKFPAAGMS RPLDTSLRLK TFSSKSEYQL VVNAVRKLQE SGFYWSAVTG GEANLLLSAE PAGTFLIRDS SDQRHFFTLS VKTQSGTKNL RIQCEGGSFS LQSDPRSTQP VPRFDCVLKL VHHYMPPPGA PSFPSPPTEP SSEVPEQPSA QPLPGSPPRR AYYIYSGGEK IPLVLSRPLS SNVATLQHLC RKTVNGHLDS YEKVTQLPGP IREFLDQYDA PL