Recombinant human HLA-DRA protein
Major histocompatibility complex, class II, DR alpha, also known as HLA-DRA, binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Recombinant human HLA-DRA, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Major histocompatibility complex class II DR alpha, Major histocompatibility complex, class II, DR alpha, HLA-DA
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
24.3 kDa (212aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
De Gassart A., et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105:3491-3496; ; Lapaque N., et al. (2009) J. Biol. Chem. 284:7007-7016;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_061984.2
Uniprot Accession Number
P01903
Species
Human
AA Sequence
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