Recombinant human HLA-DRA protein

Major histocompatibility complex, class II, DR alpha, also known as HLA-DRA, binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Recombinant human HLA-DRA, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Major histocompatibility complex class II DR alpha, Major histocompatibility complex, class II, DR alpha, HLA-DA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.3 kDa (212aa) confirmed by MALDI-TOF

Additionnal Information

HLA-DR, Major histocompatibility complex class II DR alpha, Major histocompatibility complex, class II, DR alpha, HLA-DA, ATGP2587-10 µg, ATGP2587-20 µg, ATGP2587-50 µg, ATGP2587-100 µg, ATGP2587-250 µg, ATGP2587-500 µg, ATGP2587-1 mg, ATGP2587-10, ATGP2587-20, ATGP2587-50, ATGP2587-100, ATGP2587-250, ATGP2587-500, ATGP2587-1

References & Citations

De Gassart A., et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105:3491-3496; ; Lapaque N., et al. (2009) J. Biol. Chem. 284:7007-7016;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_061984.2

Uniprot Accession Number

P01903

Species

Human

AA Sequence

IKEEHVIIQ AEFYLNPDQS GEFMFDFDGD EIFHVDMAKK ETVWRLEEFG RFASFEAQGA LANIAVDKAN LEIMTKRSNY TPITNVPPEV TVLTNSPVEL REPNVLICFI DKFTPPVVNV TWLRNGKPVT TGVSETVFLP REDHLFRKFH YLPFLPSTED VYDCRVEHWG LDEPLLKHWE FDAPSPLPET TE