Recombinant human Cathepsin E protein

CTSE is a gastric aspartyl protease that functions as a disulfide-linked homodimer. CTSE, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. Recombinant human CTSE protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Cathepsin E, CATE, CTSE

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.4 kDa (330aa)

Additionnal Information

CTSE, Cathepsin E, CATE, ATGP2586-10 µg, ATGP2586-20 µg, ATGP2586-50 µg, ATGP2586-100 µg, ATGP2586-250 µg, ATGP2586-500 µg, ATGP2586-1 mg, ATGP2586-10, ATGP2586-20, ATGP2586-50, ATGP2586-100, ATGP2586-250, ATGP2586-500, ATGP2586-1

References & Citations

Sealy L., et al. (1996) Eur. J. Immunol. 26:1838-1843; ; Cook M., et al. (2001) Eur. J. Biochem. 268:2658-2668;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_683865

Uniprot Accession Number

P14091

Species

Human

AA Sequence

TESCSMD QSAKEPLINY LDMEYFGTIS IGSPPQNFTV IFDTGSSNLW VPSVYCTSPA CKTHSRFQPS QSSTYSQPGQ SFSIQYGTGS LSGIIGADQV SVEGLTVVGQ QFGESVTEPG QTFVDAEFDG ILGLGYPSLA VGGVTPVFDN MMAQNLVDLP MFSVYMSSNP EGGAGSELIF GGYDHSHFSG SLNWVPVTKQ AYWQIALDNM LWSVPTLTSC RMSPSPLTES PIPSAQLPTP YWTSWMECSS AAVAFKDLTS TLQLGPSGSW GMSSFDSFTQ SLTVGITVWD WPQQSPKEGP CVCACLSDRP