Recombinant human HLA-DRB1 protein

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Recombinant human HLA-DRB1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Major histocompatibility complex class II DR beta 1, Major histocompatibility complex, class II, DR beta 1, DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

25.2 kDa (219aa)

Additionnal Information

HLA-DRB1, Major histocompatibility complex class II DR beta 1, Major histocompatibility complex, class II, DR beta 1, DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, ATGP2585-10 µg, ATGP2585-20 µg, ATGP2585-50 µg, ATGP2585-100 µg, ATGP2585-250 µg, ATGP2585-500 µg, ATGP2585-1 mg, ATGP2585-10, ATGP2585-20, ATGP2585-50, ATGP2585-100, ATGP2585-250, ATGP2585-500, ATGP2585-1

References & Citations

Menendez-Benito V., et al. (2007) Immunity. 26:1-3; ; De Gassart A., et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105:3491-3496;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002115

Uniprot Accession Number

P01911

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGDTRPRFLW QPKRECHFFN GTERVRFLDR YFYNQEESVR FDSDVGEFRA VTELGRPDAE YWNSQKDILE QARAAVDTYC RHNYGVVESF TVQRRVQPKV TVYPSKTQPL QHHNLLVCSV SGFYPGSIEV RWFLNGQEEK AGMVSTGLIQ NGDWTFQTLV MLETVPRSGE VYTCQVEHPS VTSPLTVEWR ARSESAQSK