Recombinant human Siglec-3/CD33 protein

CD33 is putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, CD33 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. This protein induces apoptosis in acute myeloid leukemia (in vitro) . Recombinant human CD33 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

SIGLEC-3, SIGLEC3, Sialic acid-binding Ig-like lectin 3, p67, Myeloid cell surface antigen CD33 isoform 1, Myeloid cell surface antigen CD33, FLJ00391, CD33 molecule, CD33

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.1 kDa (265aa)

Additionnal Information

CD33, CD33 molecule, FLJ00391, p67, SIGLEC-3, SIGLEC3, ATGP2584-10 µg, ATGP2584-20 µg, ATGP2584-50 µg, ATGP2584-100 µg, ATGP2584-250 µg, ATGP2584-500 µg, ATGP2584-1 mg, ATGP2584-10, ATGP2584-20, ATGP2584-50, ATGP2584-100, ATGP2584-250, ATGP2584-500, ATGP2584-1

References & Citations

Ulyanova T., et al. (1999) Eur. J. Immunol. 29:3440-3449; ; Vitale C., et al. (2001) Proc. Natl. Acad. Sci. u.S.A. 98:5764-5769;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Stem cell

NCBI Accession Number

AAH28152

Uniprot Accession Number

P20138

Species

Human

AA Sequence

DPNFWLQ VQESVTVQEG LCVLVPCTFF HPIPYYDKNS PVHGYWFREG AIISGDSPVA TNKLDQEVQE ETQGRFRLLG DPSRNNCSLS IVDARRRDNG SYFFRMERGS TKYSYKSPQL SVHVTDLTHR PKILIPGTLE PGHSKNLTCS VSWACEQGTP PIFSWLSAAP TSLGPRTTHS SVLIITPRPQ DHGTNLTCQV KFAGAGVTTE RTIQLNVTYV PQNPTTGIFP GDGSGKQETR AGVVH